rabbit polyclonal anti-human antibodies for car and cd46  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: The Local Complement Activation on Vascular Bed of Patients with Systemic Sclerosis: A Hypothesis-Generating Study

doi: 10.1371/journal.pone.0114856

Figure Lengend Snippet: Skin sections from 8 SSc patients (4 lesional SSc skin and 4 non-lesional SSc skin), 12 controls (8 healthy subjects and 4 ScGVHD patients) were immunostained with anti-CD46 (green) specific antibody. Anti-CD31 antibody (orange) was used as a marker of endothelial cells. Data of the analysed fields ( n = 20 for each slide) are expressed as ratio between MCP (CD46) positive vessels and total number of vessels.

Article Snippet: For immunofluorescence staining, sections were separately incubated with three different antibodies: a monoclonal mouse anti-human CD31 antibody (Dako, JC70A, 1:50 dilution; a marker for vascular endothelium), a monoclonal rabbit anti-human CD46 antibody (Abcam, ab108307, 1:500 dilution), and a monoclonal murine anti-human SC5b-9 antibody (QUIDEL, A239, 1:250 dilution).

Techniques: Marker

Journal: PLoS ONE

Article Title: The Local Complement Activation on Vascular Bed of Patients with Systemic Sclerosis: A Hypothesis-Generating Study

doi: 10.1371/journal.pone.0114856

Figure Lengend Snippet: Protein extract from skin biopsies of 8 SSc patients (4 lesional SSc skin and 4 non-lesional SSc skin) and 8 healthy subjects were analyzed by immunoblot using a specific antibody. Results of a representative experiment of four are shown. Data are expressed as an optical density ratio of MCP (CD46) and β-tubulin.

Article Snippet: For immunofluorescence staining, sections were separately incubated with three different antibodies: a monoclonal mouse anti-human CD31 antibody (Dako, JC70A, 1:50 dilution; a marker for vascular endothelium), a monoclonal rabbit anti-human CD46 antibody (Abcam, ab108307, 1:500 dilution), and a monoclonal murine anti-human SC5b-9 antibody (QUIDEL, A239, 1:250 dilution).

Techniques: Western Blot